Lling, serological parameters and by radiological methods. Furthermore, the effects of

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Based on its unique function and distribution it was tried to selectively eliminate inflammatory Trop. 1998;46:1?. 23. Mohammed T, Erko B, Giday M. Evaluation of antimalarial activity macrophages by a FcRI-directed humanized antibody (H22) conjugated to the toxin Ricin A (anti-FcRI-RiA). Furthermore, IL-1 production by RA synovial tissue explants was inhibited (63 , P PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27486068 invasion of fibroblast-like cells into the articular cartilage. Destruction could be reduced by methotrexate but not by cyclosporine A or FK 506, indicating a fibroblast-directed action of methotrexate connected to reduction of joint destruction in RA patients. Transfection with cytokines did not act on cartilage destruction, but IL-11 reduced apoptosis of chondrocytes. Induction of cartilage destruction by intra-articular application of murine fibroblast-like cells, in particular LS48, is a rapid and highly reproducible model for investigating invasive arthritis and can be modulated by drugs or gene transfer. This provides the opportunity to check novel therapeutic strategies for the treatment of arthritis, especially focussing on the reduction of cartilage erosion.endocytose FcRI-targeted antigens. Based on its unique function and distribution it was tried to selectively eliminate inflammatory macrophages by a FcRI-directed humanized antibody (H22) conjugated to the toxin Ricin A (anti-FcRI-RiA). FcRI expression (CD64) was determined on RA monocytes/ macrophages (mo/mac's) and granulocytes from peripheral blood (PB) and synovial fluid (SF). Anti-FcRI-RiA was tested on mo/mac's from PB and SF of 12 RA patients. Cell death of mo/mac's (vs. lymphocytes) was assessed by morphological changes, changes in CD14 expression and nuclear DNA fragmentation (measuring apoptosis) after 24 hours. The anti-inflammatory effect in vitro was tested by the effect of anti-FcRI-RiA on TNF production, antigen-induced Th1-mediated proliferation and T-cell survival in the context of SFMC. In addition the effect on cytokine production by synovial tissue explants was studied (n=6). FcRI was exclusively expressed on mo/mac's and was PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26780312 higher in SF than PB (MFI 232 vs. 59, P